BP 21 Yusra Azhar

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Contents

Location of Collection

Beaver Pond on Georgia Gwinnett College campus

Date of Collection

August 2012

Shannon-Wiener Index of Biodiversity

Not applicable for this project.

Pictures

Daphnia 1.JPG

Distinguishing Morphological Features of Order

  • Disc shaped body
  • Small size (~0.2-18 mm)
  • Single black central eye
  • Small mouth part
  • Carapace covers body and legs but not the head
  • 4-6 pairs of thoracic legs
  • Large appendages
  • Most pigmented part is usually the eggs
  • Short antennules used as sensory organs

Sub-order

Anomopoda

Family

Daphniidae

Genus

Daphnia

Species

Daphnia pulex

Geographical Distribution

North and South America, Europe and Australia.

Life Cycle

Asexual Reproduction: (Parthenogenesis) Without fertilization, all eggs develop into female daphnia

  • Egg (Unfertilized egg mature into larvae)
  • Embryo
  • Juvenile (Young hatch quickly and resemble adults)
  • Adult (Quick maturation and able to reproduce)

Sexual Reproduction: (Bisexual)

  • Eggs wrapped in epiphium (Resting eggs encased in a structure on the back of female, will stay in eggs during unfavorable conditions)
  • Eggs hatch after diapause
  • Embryo (After males fertilize resting eggs)
  • Juvenile (Fully developed neonate, undergo 4 moltings while size increases)
  • Adult (Sexually mature)

Sexual Dimorphism

Female D. pulex have larger traits in respect to carapace and antennules than males.

What it eats

Feeds on planktonic algae, diatoms, bacteria and cynobacteria.

Habitat

Found in a broad range of aquatic habitats, preferably open waters and fresh waters.

Ecological Importance

D. pulex has unique adaptability skills that are result of chemicals given off by predators or environmental distress. These can be used to test water quality and measure environmental changes.

Economic, Agricultural, & Human Health Importance

  • D. pulex are beneficial to aquatic environments: Important to aquatic food webs because they serve as major food sources to various fish.
  • Clear bodies of water from debris: Convert phytoplankton and decay matter into usable forms in freshwater ecosystem.
  • Useful in scientific experiments because they are cheap, easily accessible and maintained, and also have fast maturation rate.
  • Science models for environmental genomics (Interaction between environment and genes). They can aid in management of water resources by protecting rhem from chemicals and environment pollutants that can help secure human health.

PCR product: Gel Pictures

October 8, 2012
BP21yusraazharpcr.png
Note: Faint bands due to human error.



October 17, 2012
2Pic2BP21yusraazharpcr.png

Sequence data

  • forward (CO1)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGANNNN
NNNNNNNNNNNNNTCNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNTNNNNNTNN
NNNNNNNNNNNNNNGANNNNNNNNTNNNNANNNNNNNNNNTNNNNNNNNNNNNNNNNTN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNANNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNTNNTNNNNNNNNGANCTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN
NNNNNNNNNNNNNNNNNNNNNNNNNNNNTNNNNNTNANNNNNNNNNNNNNNNNNNNNNN
NNNNNNNTN
  • reverse (CO1)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGNNGGGNNAANNNN
NNANNNNNNNNNNNNCGANNNNNNNNNNNNNNNNAANNNNNNNNGTNNNNNNNGNNNGA
NNNNNNNNNNNNNNNGNNNNGNNNNNNNNNNNNNNNNCNNANNTNANGNAATNNNNNNT
NNNNNNNTNCCCTCNGNNNGNNTATTANNNNNNNNNNNAATNAANNNNNNNNNNNNNNN
ATNNAAGANNTCCCNNNCAAATGTAGANAAAAAATTNNANNANNNNNNNANNNNCNNNN
NNNNNNNNTTNNNNNNGANANNNNANNNNNANNNGNNCNNCCNGNNNNNNNNCNNNNTT
CTACNNNNNNNNNNNNNAAAANAANANTNNGNGNNNNNNNNNNNNNNNNNNNNNNNNNN
NTNNNTNNNNNNGNNNNNGNNNNTNTGNNNNNNNTNNNTTTNNNGNNANNNNNNNTNNN
TTNNNNNNNNNANNNTNNTGNNNANNNNTNAAANNNNNNNTNNNNNNNGNNNGNNNNNT
NNNNTNNNCNTTNNNAANNNNNNNNNNNNNNTNNNNNTNNNNNTNNTNNNNTTTNTNNN
NNNNNNNNNNNNNNNNTNTTTCTNNNNTTNNNNNNNNNNNNNNNNNANNNNNNNNNNNN
TNTTTTNTNNNNNNNTGATNATTTNNNNNTNNNNTNTANNNNNNNNTNTTNNTNNNNCN
TNATNTNNNNNNNNNNN

References

http://www.caudata.org/daphnia/
http://cfb.unh.edu/cfbkey/html/Organisms/CCladocera/FDaphnidae/GDaphnia/Daphnia_pulex/daphniapulex.html
http://animaldiversity.ummz.umich.edu/accounts/Daphnia_pulex/
http://www.ncbi.nlm.nih.gov/books/NBK2042/

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